Isolating, Cloning and Sequencing DNA (DNA Analysis)

A) PCR

  1. Polymerase Chain Reaction animation http://www.dnalc.org/resources/animations/pcr.html


B) Gel Electrophoresis; Southern Blot

  1. Gel electrophoresis animation + Virtual gel electrophoresis experiment http://learn.genetics.utah.edu/content/labs/gel/

  2. Gel electrophoresis experiment https://www.youtube.com/watch?v=3I9wzwj0b_A

  3. Southern Blot Video https://www.youtube.com/watch?v=-WsYENmDUZk

C) RFLP Analysis & Applications

  1. DNA profiling https://www.youtube.com/watch?v=7onjVBsQwQ8

  2. Genetic mapping https://www.youtube.com/watch?v=TU44tR0hJ8A

  3. Restriction Fragment Length Polymorphisms Video https://www.youtube.com/watch?v=jMLIaxxY6-8


D) DNA Sequencing

  1. DNA Sequencing animation (detailed) http://www.dnalc.org/resources/animations/cycseq.html

  2. DNA Sequencing and ddNTP animation http://www.dnalc.org/resources/animations/sangerseq.html

  3. Shotgun sequencing Video https://www.youtube.com/watch?v=t3etbc1hKLs


E) DNA Synthesis

  1. CRISPR Video https://www.youtube.com/watch?v=FNp2wR4mK1Q

  2. Oligonucleotide Synthesis Video https://www.youtube.com/watch?v=kjSojZWnPQ4