Isolating, Cloning and Sequencing DNA (DNA Analysis)

A) PCR

  1. Polymerase Chain Reaction animation http://www.dnalc.org/resources/animations/pcr.html


B) Gel Electrophoresis; Southern Blot

  1. Gel electrophoresis animation + Virtual gel electrophoresis experiment http://learn.genetics.utah.edu/content/labs/gel/

  2. Gel electrophoresis experiment https://www.youtube.com/watch?v=3I9wzwj0b_A

  3. Southern Blot Video https://www.youtube.com/watch?v=-WsYENmDUZk

C) RFLP Analysis & Applications

  1. DNA profiling https://www.youtube.com/watch?v=7onjVBsQwQ8

  2. Genetic mapping https://www.youtube.com/watch?v=TU44tR0hJ8A

  3. Restriction Fragment Length Polymorphisms Video https://www.youtube.com/watch?v=jMLIaxxY6-8


D) DNA Sequencing

  1. DNA Sequencing animation (detailed) http://www.dnalc.org/resources/animations/cycseq.html

  2. DNA Sequencing and ddNTP animation http://www.dnalc.org/resources/animations/sangerseq.html

  3. Shotgun sequencing Video https://www.youtube.com/watch?v=t3etbc1hKLs


E) DNA Synthesis & DNA Editing

  1. Simplified Introduction of CRISPR Cas9 https://www.youtube.com/watch?v=UKbrwPL3wXE

  2. Natural CRISPR-Cas9 mechanism in Bacteria (Introduction) https://www.youtube.com/watch?v=MbJ7Hnc2K3Q

  3. Natural CRISPR-Cas9 mechanism in Bacteria (Detailed) https://www.youtube.com/watch?v=MnYppmstxIs

  4. CRISPR-Cas9 in Editing Human Genome and its Ethical Issues https://www.youtube.com/watch?v=pVIVSpUgR44

  5. CRISPR-Cas9 Societal Applications https://www.youtube.com/watch?v=6tw_JVz_IEc

  6. CRISPR & Societal Implications [23 min_Video] https://www.channelnewsasia.com/watch/why-it-matters-cna/should-we-hack-our-dna-1460546

  7. CRISPR-Cas9 usage in AAV to treat eye disease https://www.youtube.com/watch?v=EPTeaXMVcyY

  8. CRISPR-Cas9 Problems: Designer Babies - The Problem With China's CRISPR Experiment https://www.youtube.com/watch?v=kFFyeHJDI50

  9. Oligonucleotide Synthesis Video https://www.youtube.com/watch?v=kjSojZWnPQ4