Isolating, Cloning and Sequencing DNA (DNA Analysis)

A) PCR

1. Polymerase Chain Reaction animation http://www.dnalc.org/resources/animations/pcr.html

B) Gel Electrophoresis; Southern Blot

1. Gel electrophoresis animation + Virtual gel electrophoresis experiment http://learn.genetics.utah.edu/content/labs/gel/

2. Gel electrophoresis experiment https://www.youtube.com/watch?v=3I9wzwj0b_A 

3. Southern Blot Video https://www.youtube.com/watch?v=vBATs6Jhk44 

C) RFLP Analysis & Applications

1. DNA profiling https://www.youtube.com/watch?v=7onjVBsQwQ8 

2. Genetic mapping https://www.youtube.com/watch?v=TU44tR0hJ8A 

3. Restriction Fragment Length Polymorphisms Video https://www.youtube.com/watch?v=jMLIaxxY6-8 

D) DNA Sequencing

1. DNA Sequencing animation (detailed) http://www.dnalc.org/resources/animations/cycseq.html

2. DNA Sequencing and ddNTP animation http://www.dnalc.org/resources/animations/sangerseq.html

3. Shotgun sequencing Video https://www.youtube.com/watch?v=t3etbc1hKLs 

4. Applications from Y chromosome sequencing_1 https://www.youtube.com/watch?v=y_ujpvJ2Hag 

5. Applications from Y chromosome sequencing_2 https://www.youtube.com/watch?v=1Wz7stz5P9c 

6. Why sequencing Y chromosome took so long https://www.youtube.com/watch?v=MB82gSy0bW0 

E) DNA Editing

1. Simplified Introduction of CRISPR Cas9 https://www.youtube.com/watch?v=UKbrwPL3wXE  

2. Natural CRISPR-Cas9 mechanism in Bacteria (Introduction) https://www.youtube.com/watch?v=MbJ7Hnc2K3Q  

3. Natural CRISPR-Cas9 mechanism in Bacteria (Detailed) https://www.youtube.com/watch?v=MnYppmstxIs 

4. CRISPR-Cas9 in Editing Human Genome and its Ethical Issues https://www.youtube.com/watch?v=pVIVSpUgR44 

5. CRISPR-Cas9 Societal Applications https://www.youtube.com/watch?v=6tw_JVz_IEc  

6. CRISPR & Societal Implications [23 min_Video] https://www.channelnewsasia.com/watch/why-it-matters-cna/should-we-hack-our-dna-1460546 

7. CRISPR-Cas9 usage in AAV to treat eye disease https://www.youtube.com/watch?v=EPTeaXMVcyY 

8. CRISPR-Cas9  Problems: Designer Babies - The Problem With China's CRISPR Experiment https://www.youtube.com/watch?v=kFFyeHJDI50 

9. Oligonucleotide Synthesis Video https://www.youtube.com/watch?v=kjSojZWnPQ4 

F) Recombinant DNA Technology

1. cDNA synthesis https://www.youtube.com/watch?v=VT_nNVO95eI

2. Blue White Selection (Introductory) https://www.youtube.com/watch?v=4zzC17RD_yE 

3. Blue White Selection (Detailed) https://www.youtube.com/watch?v=4fnS2xKjIbg 

4. Replica Plating https://www.youtube.com/watch?v=eA9Kw4d_O9Y 

5. DNA LInkers and Adaptors https://www.youtube.com/watch?v=eLCImZyA1yw  

6. GM Tomato Review https://www.youtube.com/watch?v=kv5TlkAN3z8 

7. Transgenic Atlantic salmon aquaculture review https://www.youtube.com/watch?v=Dm-0EfAAY-8 

8. Plant patenting and farming issues https://www.youtube.com/watch?v=gBX6CNfEkk4 

9. GMO Food safety concerns https://www.youtube.com/watch?v=KyLkhP1CNS0